The Molecular Diagnostics Laboratory (MDL) at MedStar Georgetown University Hospital is a comprehensive multidisciplinary division including the Immunohistochemistry (IHC), Flow Cytometry, Fluorescence In-Situ Hybridization (FISH), and Molecular operations.
The IHC section comprises a wide variety of markers used for the histogenetic diagnosis of morphologically non-differentiated neoplasias, subtyping neoplasias, and the characterization of primary site of malignant neoplasias. Offered in the IHC section as well are a set of predictive markers that contributes towards surgical and therapeutic conducts of some diseases.
The flow cytometry section is focused on Leukemia and Lymphoma Immunophenotyping using the 6-color FACS Canto platform. The distributed nature of the hematopoietic system makes it amenable to flow cytometric analysis. Many surface proteins and glycoproteins on erythrocytes, leukocytes, and platelets are tested with a variety of antibodies, in addition to antibodies against intracellular proteins such as myeloperoxidase and terminal deoxynucleotidyl transferase.
The FISH section plays an important role in detecting specific biomarkers in solid neoplasms such as ALK-1 gene rearrangement in Non-Small Cell Lung Cancer (NSCLC) and HER-2 for Breast Cancer.
The molecular operation is divided into four major sections:
- The molecular hematopathology section offering assays for the diagnosis and prognosis of lymphoid and myeloid neoplasms by capillary electrophoresis, and monitoring Minimal Residual Diseases (MRD) by Real-Time Polymerase Chain Reaction (PCR).
- The molecular oncology section offering assays for the detection of specific mutations in key genes in Lung Cancer, Colorectal Cancer, and Melanoma by Real-time PCR.
- The molecular virology section offering a long list of assays to monitor the viral loads of several infectious diseases by Real-Time using a combination of FDA-approved and analytically validated Laboratory Developed Tests (LDTs).
- The molecular genetics section offering molecular coagulation assays by Real-Time PCR.
To inquire about sending samples to our laboratory for testing please contact the Pathology Customer Service Office at (202) 784-3614.
- Joeffrey Chahine